Phosphorus forms in the sediment of seagrass meadows affected mainly by fungi rather than bacteria: a preliminary study based on 31P-NMR and high-throughput sequencing

Muqiu Zhao, Hui Wang, Shuai Wang, Qiuying Han, Yunfeng Shi

Paper category: Original research paper
Corresponding author: Yunfeng Shi (
DOI: 10.1515/ohs-2020-0036
Received: 10/04/2020
Accepted: 25/05/2020
Full text: here

Citation: Zhao, M., Wang, H., Wang, S., Han, Q., & Shi, Y. (2020). Phosphorus forms in the sediment of seagrass meadows affected mainly by fungi rather than bacteria: a preliminary study based on 31P-NMR and high-throughput sequencing, Oceanological and Hydrobiological Studies, 49(4), 408-420. doi:


Microorganisms play an important role in the circulation of phosphorus (P) in the sediment of coastal wetland ecosystems. In this study, solution <sup>31</sup>P nuclear magnetic resonance (NMR) was used to determine different forms of P in the sediments of four different seagrass meadows and a bare tidal flat, while high-throughput 16S and ITS rRNA gene sequencing was used to determine the microbial community composition. The solution <sup>31</sup>P-NMR spectra revealed six forms of the P compounds detected by the NaOH-EDTA extraction of sediments, where Ortho-P was the most dominant P compound, followed by Mono-P. The Po compounds were more varied in the seagrass meadow sediments and more abundant compared to the bare tidal flat. Bacterial communities in the sediments collected from E. acoroides and fungal communities in the bare tidal flat were relatively different from those at the other sites. The relative abundance of P-cycling-related fungi belonging to the phylum Ascomycota was 26.20% and was much higher than that of bacteria (only 0.29%) belonging to the class Bacilli. Mono-P was the major factor determining the distribution of P-cycling-related fungi and negatively correlated with the relative abundance of Aspergillus and Trichoderma. We believe that fungi can affect P forms in the sediment of seagrass meadows more than bacteria.

This work was supported by the Natural Science Foundation of Hainan Province (No. 418MS074), Young Talents’ Science and Technology Innovation Project of Hainan Association for Science and Technology (No. QCXM201811), the Key Project of Natural Science Foundation of China (No. 41730529) and the Regional Project of Natural Science Foundation of China (No. 41766004).


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